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FAO
FISHERIES
TECHNICAL
PAPER
395
Edited by
Peter Walker
CSIRO, Australia
and
Rohana Subasinghe
FAO
Report and Proceedings of the Expert Workshop on DNA-based Molecular Diagnostic Techniques: Research Needs for Standardization and Validation of the Detection of Aquatic Animal Pathogens and Diseases.
Bangkok, Thailand, 7-9 February 1999
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The designations employed and the presentation of material in this publication do not imply the expression of any opinion whatsoever on the part of the Food and Agriculture Organization of the United Nations concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries. |
ISBN 92-5-104420-1
All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying or otherwise, without the prior permission of the copyright owner. Applications for such permission, with a statement of the purpose and extent of the reproduction, should be addressed to the Director, Information Division, Food and Agriculture Organization of the United Nations, Viale delle Terme di Caracalla, 00100 Rome, Italy.
© FAO 2000
1.1 GLOBAL AQUACULTURE DEVELOPMENT
1.2 IMPACT OF DISEASE
1.3 ROLE OF DNA-BASED TECHNOLOGIES IN DIAGNOSIS AND PATHOGEN DETECTION
1.4 IMPEDIMENTS TO THE USE OF DNA-BASED DIAGNOSTIC TECHNIQUES
2.1 TERMS OF REFERENCE
2.2 PARTICIPANTS
2.3 WORKSHOP PROCESS
3 PATHOGEN FOCUS GROUP REPORTS
3.1.1 Status of research and identification of research needs
3.1.2 Standardization of DNA-based diagnostic tests
3.1.3 Networking and communication
3.1.4 Training and extension3.2 MOLLUSC PATHOGENS
3.3 FINFISH PATHOGENS3.3.1 Diseases requiring application of DNA-based technologies
3.3.2 Evaluation of needs for rapid detection techniques
3.3.3 Status of research
3.3.4 Key constraints to establishment of international standards
3.3.5 Recommendations for research programs
5 KEY OUTCOMES AND RECOMMENDATIONS
5.1 GENERAL COMMENTS
5.2 RESEARCH NEEDS
5.3 TRAINING NEEDS
5.4 COMMUNICATION NEEDS
5.5 INTERNATIONAL STANDARDISATION
7 CONTRIBUTED PAPERS AND REVIEWS
TECHNOLOGICAL CONSTRAINTS TO DISEASE PREVENTION AND CONTROL IN AQUATIC ANIMALS, WITH SPECIAL RFERENCE TO PATHOGEN DETECTION - SHARON MCGLADDERY
THE ABSOLUTE REQUIREMENT FOR PREDICTIVE VALIDATION OF NON-CULTURE BASED DETECTION TECHNIQUES - MAURA HINEY
EVALUATION OF DIAGNOSTIC TESTS: THE EPIDEMIOLOGICAL APPROACH - DANIEL F. FEGAN
DNA-BASED DIAGNOSTIC AND DETECTION METHODS FOR PENAEID SHRIMP VIRAL DISEASES - DONALD V. LIGHTNER
PRACTICAL PROBLEMS WITH PCR DETECTION IN ASIA: THE IMPORTANCE OF STANDARDIZATION - M. SHARIFF, S. SOON, K. L. LEE AND L.T. TAN
APPLICATION OF POLYMERASE CHAIN REACTION FOR DETECTION OF SHRIMP PATHOGENS IN INDIA - INDRANI KARUNASAGAR
VIRAL GENETIC VARIATION: IMPLICATIONS FOR DISEASE DIAGNOSIS AND DETECTION OF SHRIMP PATHOGENS - PETER J. WALKER AND JEFF A. COWLEY
THE NEED FOR MOLECULAR TOOLS IN THE STUDY OF MOLLUSC PATHOGENS - P.M. HINE
DEVELOPMENT AND VALIDATION OF DNA-BASED DIAGNOSTIC TECHNIQUES WITH PARTICULAR REFERENCE TO BIVALVE MOLLUSC PATHOGENS - FRANCK C.J. BERTHE
MOLECULAR DIAGNOSTICS FOR THE OYSTER PATHOGENS HAPLOSPORIDIUM NELSONI (MSX DISEASE) AND PERKINSUS MARINUS (DERMO DISEASE) IN CHESAPEAKE BAY, VIRGINIA, USA - EUGENE M. BURRESON
RAPID DETECTION AND IDENTIFICATION OF FISH PATHOGENS - ALEXANDRA ADAMS
DNA-BASED STUDIES ON APHANOMYCES INVADANS, THE FUNGAL PATHOGEN OF EPIZOOTIC ULCERATIVE SYNDROME (EUS) - JAMES H. LILLEY AND SUPRANEE CHINABUT
IMPORTANCE OF STANDARDISATION AND VALIDATION OF MOLECULAR DIAGNOSTIC TECHNIQUES FOR AQUATIC ANIMAL PATHOGENS AND DISEASES FOR INTERNATIONAL TRADE AND TRANS-BOUNDARY MOVEMENT OF AQUATIC ANIMALS AND ANIMAL PRODUCTS - BARRY J. HILL