FI: DP/HUN/79/001
Field Document 2
February 1982

THE HUNGARIAN PEOPLE'S REPUBLIC
FISH DISEASE RESEARCH

TABLE OF CONTENTS



A report prepared for the
Development of Intensive Freshwater Fish Culture Project


by


R. Kusuda
Fish Disease Consultant


This is one of a series of reports prepared during the course of the FAO/UNDP project identified on the title page. The conclusions and recommendations given in the report are those considered appropriate at the time of its preparation. They may be modified in the light of further knowledge gained at subsequent stages of the project.

The designations employed and the presentation of the material in this document do not imply the expression of any opinion whatsoever on the part of the United Nations or the Food and Agriculture Organization of the United Nations concerning the legal or constitutional status of any country, territory or sea area, or concerning the delimitation of frontiers.

SUMMARY
The fish disease consultancy for the FAO/UNDP Project HUN/79/001 was carried out from 1 to 29 July 1981 at the Fish Culture Research Institute (FCRI), Szarvas, Hungary. Major objectives of the consultancy were to review on-going research work on fish diseases; to assist the research team in formulation and initiation of expanded research work on bacterial fish diseases and immunological response of fish to bacterial infections, and introduction of methods for mass immunization.
Research work on fish diseases has rapidly been given an assured status since 1976. At present bacteriological and immunological studies of fish diseases are being initiated.
It is natural that, when freshwater fishes are cultured more intensively, they develop various diseases more frequently and the diseases become more prevalent. It is necessary to isolate and identify the pathogen of these diseases and at the same time to make studies to establish preventive and therapeutic methods against these diseases.
To protect fish from various bacterial diseases, it is first necessary to improve the technology for fish culture and to culture healthy fish which are highly resistant to diseases. Secondly, early treatment methods for fish diseases, as well as diagnostic procedures for early detection of fish diseases, should be established. Thirdly, it is necessary to make studies of artificial immunization with vaccines and to prevent diseases of cultured fishes. Then it becomes necessary to direct studies to be made at the Institute for facilitation of studies in the three above mentioned fields. For this purpose, it is recommended that qualified research workers be trained and that facilities, equipment and supplies required be provided to fully support these studies.

FOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS
Rome, 1982


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TABLE OF CONTENTS

1. INTRODUCTION

1.1 Terms of Reference
1.2 Background Information

2. EVALUATION OF THE STUDIES ON FISH DISEASE

3. CONCLUSIONS AND RECOMMENDATIONS

3.1 Inhibition of Development of Fish Diseases by Improvement of Means of Fish Culture Techniques
3.2 Establishment of Early Diagnostic Procedures
3.3 Inhibition of Development of Fish Diseases by immunization vaccines

Appendix 1: FACILITIES REQUIRED FOR FLUORESCENT DIAGNOSTIC PROCEDURE

LIST OF FIGURES

1. The rapid identification procedure for Aeromonas spp.
2. The rapid identification for Pseudomonas sp.
3. The rapid identification for Edwardsiella tarda
4. The rapid identification for Pseudomonas anguilliseptica
5. The rapid identification for Flexibacter columnalis
6. The rapid identification for Vibrio anguillarum
7. The rapid identification for Aeromonas salmonicida
8. The rapid identification for Vibrio anguillarum
9. The rapid identification for Corynebacterium sp.
10. The rapid identification for Hemophilus piscium
11. The rapid identification for Yersinia ruckeri
12. The manufacturing and immunization method of vaccines
13. The manufacturing and immunization method of vaccines (immersion vaccine)
14. The manufacturing and immunization method of vaccines (spray vaccine)
15. The manufacturing and immunization method of vaccines (challenging proced.)
16. The manufacturing and immunization method of vaccines (preparation and administration of pseudotuberculosis vaccine)