Previous Page Table of Contents

Appendix 1
LIST OF EQUIPMENT FOR UNIT FOR ICHTHYOPATHOLOGY AND FISH HEALTH PROTECTION, FARTC, KAUSALYAGANG, BHUBANESWAR

I.To be procured from UNDP  
    Quantity
  1.Cannon AE 136 mm reflex camera with 1:1.5 lens1
  2.Cannon close-up lenses up to 1:1 magnification in macrophotography, a tube attachment for micro-photography, angle viewer and stand for close-up photography1
  3.Leitz Diavert inverted microscope, as per quotation and order (with specifications)1
  4.Reichert-Jung autocut microtome, standard outfit1
  5.Temtool automatic microtome knife sharpener1
  6.Reichert heating and cooling plate HK 1201
  7.Deep freezer (-70° to -90°C) cabinet1
  8.Laminar flow sterile chamber1
  9.Table top refrigerator centrifuge (down to + 4°C) such as Beckman TF-6R1
10.Leitz Orthoplan microscope with Fluotar Brightfield and Phase contant objectives NPL Fluotar, GW Eye-pieces 10 × binocular pluto tube, wide field phase contrast condenser system, Interference contrast device Y and tracing device1
11.Field (portable) pH meter1
12.Oxygen dispensers for fish transportation tanks5
II.To be procured from CIFRI  
  1.Incubator (temperature range 40°-60°C1
  2.BOD cabinet (range + 4° to + 40°C)2
  3.Refrigerators 280 1 (Kelvinator)2
  4.Hood (sterile chamber)2
  5.Magnetic stirrer (urgent)1
  6.Slide cabinet for 2 000 slides2
  7.Laboratory pH meter1
  8.Hot-air oven with thermostat and clock adjustment (ambient to 200°C)1
  9.Domestic pressure cooker 20 11
10.Plant for triple glass distillation of water1
11.Fish transportation tank, 1 m3 volume with sleeve for fish unloading1
12.Fish transportation tank, 0.5 m3 volume with sleeve for unloading4
13.Oxygen cylinders2
14.Pressure reducers for oxygen cylinders, with manometers (regulators)2
15.Sieves for fish sampling with leads, floats and ropes2
16.Table cabinet for protection of fluorescent microscope from moisture and dust1
17.Automatic slide projector1
18.Cylinders with carbon dioxide for freeze microtone2
19.Butane gas cylinders for providing gas flame in laboratories3

Appendix 2
DATA SHEET FOR FISH ORGANS SAMPLED FROM MARKET

Date
SpeciesWeight
SourceMarket
Serial number of sample  
Macroscopic observations:  
  1 Grade of freshness  
         (1 - very fresh  
          2 - general condition  
          3 - clinical symptoms, if any  
          4 - started decaying)  
Material taken:  
  1 For histology  
  2 For Parasitology  
  3 For Virology  
Findings:  
  1 Histopathological alterations in liver  
  2 Histopathological alterations in kidneys  
  3 Myxosporidia in wet mounts  

    -++++++Other parasites
Oval spores:Kidney          
Liver          
Round spores:Kidney          
Liver          

4 Virological examinations

Appendix 3
DATA SHEET ACCOMPANYING FISH SENT FOR HEALTH EXAMINATION DURING REGULAR SAMPLING

1.WATER BODY
1.1Name of the pond/No. of the pond
1.2Observations on any deviation from normal water quality
2.SAMPLES
2.1Catla:No. of specimens-age
2.2Rohu:No. of specimens-age
2.3Mrigal:No. of specimens-age
2.4Silver carp:No. of specimens-age
2.5Grass carp:No. of specimens-age
2.6Common carp:No. of specimens-age

REMARKS

Bimonthly sampling should be followed throughout the year. Samples should be taken from 2 to 3 most densely stocked ponds. Thirty specimens of each species are to be sent in live or freshly dead condition. The laboratory should be notified at least one day before the arrival of samples. Diagnosis and recommendations for treatments (if necessary) will be sent officially not later than within seven days.

Appendix 4
CASE HISTORY DATA TO ACCOMPANY SICK FISH SHIPMENT FOR LABORATORY EXAMINATION

1.WATER BODY
1.1Name of pond, river or reservoir
1.2Water quality (apparently normal, altered smell or colour, algae bloom, lack of oxygen suspected, others ….)1
1.3Water sample taken for analysis and sent
2.FISH (INCLUDING EGGS OR LARVAE)
2.1Species and sizes affected by disease
2.2Unaffected species and sizes in same water
2.3Stress factors prior to disease outbreak (netting, stocking, sudden change in water temperature or oxygen concentration, others ….)1
3.DISEASE
3.1Date and hour when disease or mortality was first noted
3.2Number of dead fish so far …. % ….
3.3Behaviour of sick fish (restlessness, loss of balance, resting near surface or bank, loss of appetite, itching or flashing, others ….)1
4.SAMPLES
4.1Number of fish samples
4.2Number of fish in each sample
4.3Food samples
4.4Water samples
4.5Date and hour of taking sample
4.6Date and hour of sending samples

1 Underline what applies

REMARKS

The laboratory should be notified as soon as possible about sending of samples. Diagnosis and recommendations for treatment will be sent as soon as possible by the quickest means and an official letter with these data will be sent not later than within seven days.

Appendix 5
Questionnaire on Extent of losses in fish culture operations and on role of diseases in losses

1.Broodfish
1.1Number of broodfish available for spawning 
1.2Number of broodfish that spawned 
1.3Loss of broodfish immediately after spawning 
1.4Loss of broodfish between two spawning seasons 
1.5Suspected causes of losses (give approximate percentage)
1.5.1Improper management 
1.5.2Poaching 
1.5.3Predators 
1.5.4Diseases caused by parasites (name if possible) 
1.5.5Other diseases (name if possible) 
1.5.6Other (specify) 
1.5.7Unknown 
1.6Symptoms of diseases observed  
1.6.1Emaciation 
1.6.2Parasites 
1.6.3Dropsy condition 
1.6.4Blindness 
1.6.5Any other (specify) 
 
2.Larvae to fry rearing
2.1Total number of larvae stocked 
2.2Stocking density (larvae/ha) 
2.3Total number of fry fished out 
2.4Suspected causes of losses  
2.4.1Improper management 
2.4.2Poaching 
2.4.3Predators 
2.4.4Parasites (Diseases caused by) 
2.4.5Other diseases 
2.4.6Other (Specify) 
2.4.7Unknown 
 
3.Fry to fingerling rearing
3.1Total number of fry stocked 
3.2Stocking density (fry/ha) 
3.3Total number of fingerlings fished out 
3.4Suspected causes of losses 
3.4.1Improper management 
3.4.2Poaching 
3.4.3Predators 
3.4.4Parasites (Diseases caused by) 
3.4.5Other diseases 
3.4.6Other (Specify) 
3.4.7Unknown 
 
4.Rearing from fingerling to market size
4.1Total number of fingerlings stocked 
4.2Stocking density (fingerlings/ha) 
4.3Total number of fish harvested 
4.4Suspected causes of losses
4.4.1Improper management 
4.4.2Poaching 
4.4.3Predators 
4.4.4Parasites (Diseases caused by) 
4.4.5Other diseases 
4.4.6Other (Specify) 
4.4.7Unknown 

Previous Page Top of Page