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ANNEX 4. GLOSSARY OF TERMS


AFLP

“Amplified Fragment Length Polymorphism” (Vos et al. 1995) involves the use of “restriction enzymes” to create DNA fragments, which are then amplified using PCR (qv) techniques and visualized on polyacrylamide gels either through radiographic or fluorescence methodologies.



c-DNA

DNA synthesized from an RNA template (as happens during normal cell division)



d; Dj; theta statistics

Statistics used in the estimation of genetic distance (Gregorius 1984)



Effective population size (Ne)

Size of an idealized population that would have the same genetic properties as those observed for the real population.



FST; GST

Measures of population sub-division, expressing the reduction of heterozygosity within sub-populations, relative to expected herterozygosity in a population without sub-divisions (Nei 1978).



Heritability

The proportion of phenotypic variance attributable to genetic variance, or the extent to which genetic individual differences contribute to individual differences in observed behavior



Isozymes

Any form of an enzyme encoded by a single genetic locus



Microsatellites

Microsatellites, alternatively known as simple sequence repeats (SSRs), short tandem repeats (STRs) or simple sequence length polymorphisms (SSLPs), are tandem repeats of DNA units generally less than 5 base pairs in length (Bruford and Wayne, 1993). Variation results from differences in the number of repeat units. Microsatellites are amplified using PCR techniques and visualized on polyacrylamide gels either through radiographic or fluorescence methodologies



Ne

See “Effective population size”



PCR

“Polymerase Chain Reaction” - a method for generating unlimited copies of any fragment of DNA (Mullis, 1990)



QTL

Quantitative Trait Locus” - a polymorphic locus which contains alleles that differentially affect the expression of a phenotypic trait



RAPD

“Randomly Amplified Polymorphic DNA” (Williams et al. 1990).



SSR

See “Microsatellites”



Theta statistics

See “d; Dj


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