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Appendix 1

Preparation of 10% neutral buffered formalin

Mix one part 1 formalin (40% formaldehyde solution) with 9 parts of phosphate buffered saline pH 7,6.



Appendix 2

Preparation of phosphate buffered saline (calcium, magnesium free)

Sodium chloride

8.00 g

Potassium chloride

0.20 g

Sodium hydrogen orthophosphate

1.15 g

Potassium dihydrogen ortophosphate

0.20 g

Dissolve in pure water and make up to 800 ml.



Appendix 3

Transport medium

Phosphate buffered saline pH 7,6 (PBS) containing antibiotics and fungizone - GLYCEROL SHOULD NOT BE INCLUDED because it is virucidal for rinderpest virus.



Appendix 4

Field record sheet

TO BE COMPLETED BY FIELD OFFICER



Appendix 5

Packaging



Appendix 6

Label for submitting samples




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