The importance of agroecological-based management practices has been widely discussed in the literature. The relationship between those practices and the regulatory services in agroecosystems is an essential contribution to the development of sustainable production systems. This paper analyzes the relationship between agroecological management practices in horticulture and the different regulation services at the farm scale. The aim is to establish a conceptual framework that facilitates technicians, researchers and managers to advance in technological alternatives that promote agroecological transition processes. Knowledge of the interactions between practices and services is necessary for decision making regarding the management of agroecosystems and the conservation of biodiversity, particularly in agricultural systems.