An 87-page training manual, entitled "Mutant germplasm characterization using molecular markers: A manual", is now available on the web. This manual on selected molecular marker techniques was prepared by the Plant Breeding and Genetic Section of the joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture using the handouts and other materials distributed to participants of the 1st FAO/IAEA Interregional Training Course on "Mutant germplasm characterisation using molecular markers" held in Seibersdorf, Austria on 1-25 October 2001. The following techniques are covered: genomic DNA isolation, restriction analysis of genomic and plasmid DNA, gel electrophoresis, Southern transfer of genomic DNA, non-radioactive DNA hybridization, silver staining, RFLP, AFLP, SSR, ISSR and RAPD analysis, and retrotransposon-based marker systems. Each chapter starts with a short description of the method followed by a detailed stepwise protocol. See http://www.iaea.org/programmes/nafa/d2/public/d2_pbl_1_6.html or contact [email protected] to request a free copy.

06/01/2005