The main aim of this study was to evaluate SOC sequestration, the national distribution, and the results with different sustainable soil management (SSM) practices. SOC sequestration potential after the adoption of SSM practices under specific conditions was expressed in different ways depending on the definition of SOC baseline stocks and time towards a new equilibrium state. The study was developed by applying the FAO approach (FAO, 2020). The main result contained a SOC potential sequestration map for Colombia at 1 km2 spatial resolution using the best available national data. The institutions involved in the process were: Colombian Corporation for Agricultural Research – AGROSAVIA, Institute of Hydrology, Meteorology and Environmental Studies – IDEAM, Agustin Codazzi Geographic Institute – IGAC, Ministry of Environment and Sustainable Development of the Republic of Colombia – MADS, Ministry of Agriculture and Rural Development of the Republic of Colombia – MADS, Ministry of Foreign Affairs of the Republic of Colombia, and Food and Agriculture Organization of the United Nations – FAO, Office in Colombia.