Biosecurity measures in beekeeping are all the operational activities implemented by beekeepers to reduce the risk of introduction and spread of specific honeybee disease agents. Biosecurity measures can differ between different regions due to local factors such as climatic conditions, beekeeping equipment used, or bee races, and the prevalence, virulence and importance of the honeybee diseases. The adoption of BMBs is the foundation of all disease control programmes, irrespective of the animal species. If biosecurity measures are properly implemented, it is possible to reduce the incidence of disease and, hence the need to apply treatments, to an absolute minimum. This entry provides a list of biosecurity measures for Varoosis, American foulbrood, European foulbrood, Nosemosis and Aethinosis. The list reported below represents one of the two annexes of the manual "Good beekeeping practices. Practical manual on how to identify and control the main diseases of the honeybee (Apis mellifera)"