Tomato production can be time consuming especially when it is done to produce and keep small amounts of seeds from several varieties. It is nevertheless important to produce and preserve seeds from interesting varieties which are well adapted to your production system and environment. In a trial conducted over two seasons at FiBL in Switzerland, two harvest regimes were tested on eight tomato genotypes. One regime consisted of a regular harvest of fruits twice a week followed by seed extraction, while the other consisted of a harvest of fruits every three weeks for seed extraction. The main difference between the fruits from the two regimes is their stage of maturity i.e. all fruits are at the optimal stage for consumption with the frequent harvest while fruits are at various maturity stage – from optimal to overripe – with the less frequent harvest.