The European Farm-to-Fork strategy considers organic farming as a core element for improving agricultural sustainability, due to manifold best practice examples for sustainable farm management. Conversion to organic farming requires comprehensive knowledge about organic production practices, and good understanding of the conversion process itself and the related rules and regulations. Encounters between farmers, researchers, advisors, and other key actors who work on organic or conversion to organic, provide opportunities to exchange and disseminate knowledge, best practice examples, and innovative ideas. These help meet farmers’ needs in the process of conversion, and overall, contribute to building an innovative ecosystem that stimulates cross-fertilisation, co-creation, and scaling up organic farming. Therefore, this EIP-AGRI Workshop “Conversion to organic farming: Innovative approaches and challenges” was designed to create conditions for exchanging knowledge, and for sharing innovative ideas and inspirational practices that support farmers in successful conversion to organic farming. The workshop made an important contribution to strengthening the European Agricultural Knowledge and Innovation System (AKIS), by building and exchanging knowledge on the conversion process. The workshop was designed as a multi-stakeholder event. Participation was based on an open call, and 74 participants from 21 European countries attended the event. The Tuscany region was chosen to host the workshop because of its suitability to demonstrate practical experiences about a biodistrict initiative. The Fiesole biodistrict, in the north of Florence, provided best practice examples of cooperation among farmers. It also offered the opportunity to gain insights into networking activities among various stakeholders along the organic value chain.