Livestock sector and One Health-related policies and laws provide the overarching framework that guides the development and transformation of the livestock sector. Local authorities, and in particular frontline animal health officers, are responsible for their implementation. To perform their function properly, frontline animal health officers should not only be technically competent but also adequately knowledgeable about these policies and laws. We interviewed 209 frontline animal health officers about their working modalities and livestock sector relevant policy and law awareness in two largely urban and peri-urban sub-regions of each Ethiopia, Kenya and Uganda, not far from the capital cities. On average, 60 percent of officers could quote at least one livestock law and 44 percent have heard of the One Health approach. There were great differences between countries, awareness on policies, laws and One Health were lowest in Ethiopia, followed by Kenya and highest in Uganda. We also found that in Ethiopia, Kenya and Uganda 22, 14 and 4 percent of officers have never recieved any training since they started working. A Poisson model shows that participating in trainings is associated with an increase in the number of policies or laws and officer can quote. A logit model suggests that participating in trainings increases the odds of an officer having heard of One Health.