Tsetse flies (Glossina spp.) infest over 11 million sq km of Africa, and are vectors of Trypanosomosis (or Trypanosomiasis) in both humans and domestic livestock. For example, it is estimated that tsetse cover over 7 percent of Zimbabwe and 60 percent of Tanzania, and Trypanosomosis has an important negative impact on livestock production in these areas. In addition to the use of trypanocidal drugs, the control of Trypanosomosis has been tackled largely by control of the tsetse fly vector. A range of techniques are available, each with advantages and disadvantages. Methods include aerial spraying, sterile insect techniques, insecticide treated cattle, and odour bait traps, and targets. Therefore, a set of simple step-by-step instructions is presented of how to assemble an Epsilon trap for tsetse, adapted and tested for use in Botswana.