FAO defines biosecurity as a strategic and integrated approach to analyzing and managing risks to human, animal and plant life and health, and associated environmental risks to the environment. Strengthening biosecurity is one of the key thematic components of the One Health priority programme area, which seeks to prevent and contain the rising losses to agricultural production and adverse ecosystem effects, under the FAO Strategic Framework’s aspiration of “Better Production”. The FAO Progressive Management Pathway for Terrestrial Animal Biosecurity (FAO-PMP-TAB) is a collaborative, stepwise approach to assist countries, industries and producers in assessing and managing biological risks at enterprise, community and national levels. It is supported by the provision of appropriate tools with shared public-private responsibilities; planning of policies, laws, regulations, institutional frameworks, guidelines and field interventions. Biosecurity is improved along terrestrial animal value chains from production up to and including the point of slaughter. Activities are developed and implemented through public-private partnerships and dialogue between different stakeholders, including governments, industry, producers and other value chain actors. Ultimately, a better and sustainable biosecurity system will contribute to enhanced community resilience, sustainable terrestrial animal sector(s) and One Health outcomes.