GLI cotton is the result of conventional breeding, using transgenic varieties LLCotton25 and GHB811. LLCotton25 contains a single copy of the cassette with the bar gene, originating from Streptomyces hygroscopicus, which expresses the enzyme PAT (phosphinothricin-N-acetyl transferase), capable of conferring tolerance to the herbicide glufosinate ammonium. This gene is expressed from the p35S promoter and contains the NOS3 terminator. GHB811 contains a cassette with the 2mepsps gene (originating from maize), which expresses the 2mEPSPS protein or 5-enolpyruvylshikimate3-phosphate synthase and confers tolerance to the glyphosate herbicide, and the hppdPfW336-1Pa gene (originating from Pseudomonas fluorescens), which expresses HPPD W336 or 4-hydroxy-phenyl-pyruvate-dioxygenase, conferring tolerance to HPPD (4-hydroxyphenyl-pyruvate-dioxygenase)-inhibiting herbicides. Events were obtained via the Agrobacterium tumefaciens route. Furthermore, these same events were combined into the GLITP event, which also contains genes that confer insect resistance. All three events mentioned, LLCotton25, GHB811 and GLITP, were analyzed by CTNBio, which decided that they do not pose a non-negligible risk to human, animal, plant or environmental health. Likewise, the two GLI parenting events have already been approved in several countries, such as Argentina, Australia, the United States and the European Union. The GLI event, in turn, has already been approved in Japan, Singapore and the Philippines.

Molecular traddicional methods.