The City Region Food Systems (CRFS) programme is a global initiative of FAO and RUAF that aims to support national and local governments and local food actors to build resilient city region food systems by strengthening rural–urban linkages and improving the resilience to climate and pandemic risks. This Handbook is designed for technical staff in local and national institutions, and practitioners in cities/city regions that are implementing the city region food system (CRFS) assessment and planning process. Its purpose is to help familiarizing with the CRFS approach and key terminology, and guide the implementation of an assessment and planning following different steps and modules. The Handbook is intended to be used in conjunction with the online Toolkit, which contains supplementary explanations, how-to tools, training materials, technical examples, and workshop resources related to activities within each project module. The Toolkit can be accessed on the CRFS programme website.