APPENDIX I

Table 1

Vitamin Activities in Blood of Selected Species

				Transketolase Activity¹
Plasma	Plasma	Species	Fish No.	Wt (g)	Hematocrit,%	Pentose GPT²	Hexose Vit.³
Clarias	1	NV	40.0	2156	142	NV	NV⁴
	2	NV	40.1	1876	105	NV	NV
	3	NV	30.0	1261	82	NV	NV
	4	220	36.8	1133	144	200	1.57
	5	240	40.2	1802	58	246	2.32
	6	240	39.8	1179	255	250	2.27
	7	240	42.5	3521	465	NV	2.00
			38.49	1567	146	232	2.04
			±3.79	±394	±39	±23	±0.30
Rohu
	1	500	40.5	1341	NV	408	2.88
	2	520	35.5	1171	NV	328	3.26
	3	650	NV	858	5753	NV	NV
	4	820	38.0	1267	NV	324	6.70
	5	850	NV	785	4912	NV	NV
	6	930	41.3	1380	NV	376	3.99
			38.95	1134	5322	359	4.21
			±2.42	±231	±420	±35	±1.49
Sand Goby
	1	360	34.6	1094	179	195	2.27
	2	400	31.0	1724	82	244	1.73
	3	440	37.0	1098	92	256	2.22
	4	440	28.5	1011	65	218	2.38
			32.78	1232	104	228	2.15
			±3.26	±286	±44	±24	±0.25
Tilapia
	1	170	32.7	1444	142	314	2.81
	2	185	31.0	816	170	325	2.82
	3	193	27.5	481	NV	376	2.48
	4	300	31.8	952	92	228	3.35
	5	320	27.7	910	132	324	3.30
	6	320	19.0	581	147	323	1.69
	7	345	31.2	370	96	311	1.37
	8	350	31.0	859	469	NV	NV
	9	350	34.6	957	1135	NV	NV
	10	330	32.2	1100	66	330	3.03
			31.08	847	339	317	2.60
			±2.14	±237	±330	±39	±0.68

¹ Transketolase activity expressed as : u moles pentose disappearance/h/ml blood
u moles hexose formed/h/ml blood.
² Glutamate-pyruvate transaminase (GPT) activity expressed as units/1 plasma
³ Vitamin C expressed as mg/100 ml plasma
⁴ NV - no value

Vitamin Activieies in Blood of Sepecies contd.


				Transketolase Activity
Plasma	Plasma	Species	Fish No.	Wt (g)	Hematocrit,%	Pentose GPT	Hexose Vit.C
Snakehead	1	450	47.0	3692	272	279	2.49
	2	485	59.8	2137	544	279	2.16
	3	850	49.1	1577	436	289	2.22
			51.97	2469	417	232	2.29
			±5.6	±895	±112	±5	±0.14
Pangasius
	1	900	41.8	385	520	NV	NV
	2	1100	38.0	768	447	NV	NV

Table 2

Composition of Common Thai Feedstuffs


Feedstuff	% Dry Matter	% Dry Matter
Feedstuff	% Dry Matter	Crude Protein	Crude Fat	Crude Fibre	Ash	NFE	Baht/kg
Broken rice	87.3	10.2	1.9	1.4	1.2	85.3	3.10
Maize	87.8	10.0	4.5	2.8	2.4	80.3	2.75
Sorghum	85.4	11.1	3.7	6.1	4.1	74.8	3.00
Cassava meal	87.7	4.6	1.1	3.8	6.5	84.0	1.75
Rice bran, raw	90.0	14.0	15.2	12.7	13.5	44.6	1.80
Rice bran, solvent extracted	94.3	14.9	1.3	21.8	22.1	39.9	2.00
Soyabean, full fat	87.9	40.5	25.3	6.2	5.8	22.2	8.50
Soyabean meal	88.5	54.2	1.2	5.3	7.9	31.4	8.30
Cottonseed meal	91.6	45.4	1.3	14.5	11.4	27.4	6.50
Sesame seed meal	90.1	51.0	1.3	6.8	12.9	28.0	7.00
Groundnut meal	90.0	49.0	8.2	8.7	5.9	28.2	7.00
Copra meal	94.0	24.6	7.2	28.4	7.4	32.5	4.00
Kathin meal	90.1	18.9	2.8	24.6	11.4	42.4	2.70
Fish meal	93.1	57.0	8.5	2.1	25.0	7.4	10.00
Meat meal	93.0	54.3	10.6	2.2	30.5	2.4	NP¹
Feathermeal	93.0	91.7	3.2	1.3	3.8	0.0	5.00
Shrimp meal	90.0	49.9	3.3	12.5	30.2	4.1	NP
Brewers grains	93.1	29.8	9.5	12.1	2.6	46.0	NP
Yeast	93.0	51.5	2.6	2.5	8.6	34.8	NP
Molasses	85.0	5.4	0.3	9.9	10.3	74.0	1.50
Bone meal	97.0	11.5	9.4	1.7	77.4	-	5.00
Limestone powder	99.0	contains 38.0% calcium					1.00
dl-Methionine		contains 98% dl-Methionine					100.00
l-Lysine		contains 98% l-Lysine					120.00
Salt							1.50

¹ No price obtained

Table 3

Test Diets for Clarias Fry¹


Ingredients	% Protein
Ingredients	40		35
Soyabean meal	18.5	28	10.5	20
Peanut meal	18.5	28	10.5	20
Fish meal	45	30	45	30
Rice bran	17	13	33	29
Vitamin and mineral premix ²	1.0	1.0	1.0	1.0
Baht/kg	7.51	7.39	6.64	6.52

¹ Most Diet Preparation
Mix all finely ground ingredients, exept rice bran; cook rice bran in water equal to 50% weight of total diet; blend dry mixture into cooked rice bran extrude through Hobart meat mincer; store pellets in refrigerator until ready to feed proximate analysis on each diet.

² Vitamin ½; mineral premix to contain the following (per gram premix):

Vitamin A (IU)	1 000
Vitamin D₃ (IU)	100
Vitamin E (IU)	10
Vitamin K	1 mg
Choline	55 mg
Niacin	20 mg
Riboflavin	4 mg
Pyridoxine (B₆)	4 mg
Thiamine	4 mg
Calcium pantothenate	10 mg
Biotin	10 mg
Folic acid	1 mg
Ascorbic acid	40 mg


FeSO₄ 7H₂O	50 mg
MnSO₄ H₂O	30 mg
ZnSO₄ 7J₂O	70 mg
Cu SO₄ 5H₂O	6 mg
Kmo₃	0.2 mg
Na₂SeO₃	0.04 mg

Table 4

Test Diets for Clarias Fingerlings¹


Baht/kg Ingredients					% Protein
Baht/kg Ingredients		30			28			25
7.5	Soyabean meal	15	24.5	27.3	11.7	21.1	24	6.7	16.1	19.1
7.0	Peanut meal	15	24.5	27.3	11.7	21.2	24.1	6.7	16.1	19.2
9.5	Fish meal	25	10	5	25	10	5	25	10	5
1.3	Rice bran	43.5	38.3	36.5	50.1	45	43	60.1	55.1	52.8
4.0	Bone meal	-	1.0	2.0	-	1.0	2.0	-	1.0	2.0
0.4	Salt	0.5	0.7	0.9	0.5	0.7	0.9	0.5	0.7	0.9
25.0	Vitamin and mineral premix²	1.0	1.0	1.0	1.0	1.0	1.0	1.0	1.0	1.0
	Baht/kg	5.58	5.40	5.42	5.23	5.12	5.0	4.68	4.57	4.53

¹ See footnote 1 in Table 2
² See footnote 2 in Table 3

Table 5

Diet Formulations for Studying Substitution of Fish Meal with Soyabean Proteins


Ingredients	Diet
Ingredients	1	2	3	4
Soyabean meal	25	-	-	-
Whole soyabean	-	80	-	60
Fish meal	30	5	50	15
Rice bran	43.5	10	49	22
dl-Methionine	-	10	-	1
Dicalcium PO	-	2.0	-	-
Salt	0.5	11.0	-	1.0
Vitamins and Mineral
Premix¹	1.0	1.0	1.0	1.0
Protein	31.2	31.7	30.9	31.1

¹ See footnote 2, Table 3 for composition

Table 6

Diets for Proposed Study on Selenium Supplementation of Diets containing Vegetable Protein as the Major Protein Source


Ingredients	Diet
Ingredients	1	2	3	4
Full fat soyabean	80	80	60	60
Fish meal	-	-	15	15
Rice bran	14	14	22	22
dl-methionine	2	2	1	1
Dicalcium phosphate	2	2	-	-
Salt	1	1	0.5	0.5
Vitamin and mineral premix	1¹	2²	1¹	1²

¹ See footnote 2, Table 3 for composition
² Same as for Diets 1 and 3, except that Na₂SeO₃ is omitted