Step 1: The tool is introduced to relevant stakeholders.

Step 2: Each of the four sectors (public health, animal/livestock health, wildlife (inclusive of both the wildlife disease and wildlife management and conservation sub-sectors), and environment) answers yes/no questions about 10 One Health indicators and the extent to which the indicators contribute to delivering the six Action Tracks of the One Health Joint Plan of Action. This step is undertaken by each sector, independent of the other sectors. It ideally takes a whole-of-society approach, and should include government representatives, academia, non-governmental organizations, civil society and the private sector.

Step 3: A facilitated workshop with representatives from the responsible Ministries, sectors and stakeholders, is held to agree on final national scores for each of the 10 indicators, the extent to which these indicators contribute to the six action tracks of the One Health Joint Plan of Action, and to complete the Next Steps and Action Plan worksheet to address gaps and identifies activities to strengthen One Health implementation at country level.

The set of activities and interventions are components of a One Health action plan with inputs from all four sectors to ensure priority areas of all sectors are included and agreed upon.

The tool allows countries to establish a baseline assessment of national One Health implementation, helps track progress on One Health implementation, and supports the roll-out of the One Health Joint Plan of Action and benchmarking for possible funding opportunities, including the Pandemic Fund.