Table 4. Formats for directory/catalogue for mulberry germplasm
Name of variety |
Accession number |
Donor name |
National accession number |
Morphology |
|
Branching nature |
Leaf color |
Curve or straightness of the branch |
Leaf surface |
Color of young shoot |
Leaf texture |
Color of mature shoot |
Leaf apex |
Phyllotaxy |
Leaf margin |
Stipule nature |
Leaf base |
Stipule duration |
Leaf Shape |
Lenticel density/sq.mm |
Leaf length (cm) |
Leaf lobation type |
Leaf width (cm) |
Leaf lobation number |
Petiole length (cm) |
Leaf nature |
Petiole width (cm) |
Anatomical |
|
Stomatal size (sq.µm) |
Palisade:spongy ratio |
Stomatal frequency (no./sq.mm) |
Upper cuticle thickness (µm) |
Idioblast length (µm) |
Lower cuticle thickness (µm) |
Idioblast width (µm) |
Lower epidermal thickness (µm) |
Idioblast frequency (no./sq.mm) |
Upper epidermal thickness (µm) |
Palisade thickness (µm) |
Leaf thickness (µm) |
Spongy thickness (µm) |
No. of chloroplast/stomata |
Reproductive |
|
Sex |
Stamen length (mm) |
Male inflorescence length (cm) |
Anther length (mm) |
Female inflorescence length (cm) |
Pollen diameter (µm) |
Bisexual inflorescence length (cm) |
Pollen viability (%) |
Male inflorescence width (cm) |
Style length (mm) |
Female inflorescence width (cm) |
Stigma length (mm) |
Bisexual inflorescence width (cm) |
Stigma type |
No. of flowers/catkin (Male) |
Stigma nature |
No. of flowers/catkin (Female) |
Fruit length (cm) |
No. of flowers/catkin (Bisexual) |
Fruit width (cm) |
Male inflorescence peduncle length |
Fruit weight (gm) |
Female inflorescence peduncle length |
Fruit color |
Bisexual inflorescence peduncle length |
Fruit taste |
Growth |
|
Budding time |
First leaf opening time |
Budding rate(%) |
Leaf yield(g)/shoot(m) |
Leaf number/kg(spring) |
Leaf number/kg(autumn) |
No. of branches/plant |
Lamina index (%) |
Longest shoot length (cm) |
Leaf petiole ratio by length |
Total shoot length (cm) |
Leaf petiole ratio by weight |
Internodal distance (cm) |
Leaf yield/plant (kg) |
Leaf yield(kg)/Mu |
Cocoon yield(kg)/100kg leaf |
Hundred leaf weight (gm) |
Leaf shoot ratio |
Leaf area (sq. cm) |
Moisture content (%) |
Lamina weight (gm) |
Moisture content after 6 hours (%) |
Petiole weight (gm) |
Moisture retention capacity (%) |
Biochemical |
|
Chlorophyll-a |
Protein soluble (dry weight) |
Chlorophyll-b |
Protein soluble (dry weight) |
Total Chlorophyll |
Carbohydrate soluble (fresh weight) |
Chlorophyll-a/b |
Carbohydrate soluble (dry weight) |
Disease |
|
Powdery mildew |
Bacterial blight |
Leaf spot |
|
Propagation |
|
Survival % |
Shoot diameter (cm) |
No. of Leaves/saplings |
Total fresh biomass/sapling (gm) |
Leaf length (cm) |
Total dry biomass/sapling (gm) |
Leaf width (cm) |
No. of roots/sapling |
Fresh leaf weight/sapling (gm) |
Fresh root weight/sapling (gm) |
Dry leaf weight/sapling (gm) |
Dry root weight/sapling (gm) |
Shoot length (cm) |
Longest root length/sapling (cm) |
Fresh shoot weight/sapling (gm) |
Root volume/sapling (ml) |
Dry shoot weight/sapling (gm) |
|
Table 5. Investigation table of silkworm rearing, seed preparation, reeling and chemical analysis of mulberry
Mulberry variety: Tree years: Cut type:
|
Parameters |
Year 20- |
Year 20- |
||
Rearing results |
Rate of survival (%) |
Spring |
Autumn |
Spring |
Autumn |
Died larva in cocooning (%) |
|
|
|
|
|
Cocoon weight (kg/10000 larvae) |
|
|
|
|
|
Cocoon number per kg |
|
|
|
|
|
Cocoon weight(g) |
|
|
|
|
|
Shell weight(g) |
|
|
|
|
|
Shell ratio(%) |
|
|
|
|
|
Moultism ratio |
|
|
|
|
|
Egg-production results |
Number of healthy/fertilized eggs |
|
|
|
|
Number of unhealthy or unfertilizrd eggs. |
|
|
|
|
|
Total egg number |
|
|
|
|
|
Egg color uniform |
|
|
|
|
|
Egg weight (g/10000eggs) |
|
|
|
|
|
Chemical analysis |
Water content (%) |
|
|
|
|
Dried material (%) |
|
|
|
|
|
Crude protein content (%) |
|
|
|
|
|
Crude lipid (%) |
|
|
|
|
|
Crude cellulose (%) |
|
|
|
|
|
Soluble carbohydrate (%) |
|
|
|
|
|
C/N |
|
|
|
|
|
Reeling results |
Cocoon weight (g) |
|
|
|
|
Shell weight (g) |
|
|
|
|
|
Filament length (m) |
|
|
|
|
|
Reelable filament length (M) |
|
|
|
|
|
Reelability(%) |
|
|
|
|
|
Filament size (d) |
|
|
|
|
|
Size deviation |
|
|
|
|
Table 6. Investigation of morphology of mulberry germplasm
Variety |
Name |
Number |
||
Origin |
District |
Pruning type |
||
Outline |
Height |
|
||
Tree type |
Type |
|
||
Shoot number |
|
|||
Shoot length |
|
|||
Stem |
Curve or straight |
|
||
Color |
|
|||
Tree skin |
|
|||
Shoot |
Type |
Curve or straight |
|
|
Stand or bending |
|
|||
Size |
|
|||
Length |
|
|||
Side shoot number |
|
|||
Internode |
Length |
|
||
Curve or straight |
|
|||
Skin color |
|
|||
Skin veins |
|
|||
Stoma ostiole |
Morpha |
|
||
Size |
|
|||
Protruding |
|
|||
Distribution |
|
|||
Number |
|
|||
Winter bud |
Morpha |
|
||
Growth character |
|
|||
Size |
|
|||
Color |
|
|||
Bud scale |
Number |
|
||
Layers |
|
|||
Accessory bud number |
|
|||
Bud bract |
|
|||
Leaf rank |
|
|||
Leaf scar |
Morpha |
|
||
Rhombic |
|
|||
Size |
|
|||
Young shoot |
Size and length |
|
||
Number |
|
|||
Color |
|
|||
Trichome |
|
|||
Blade |
Morpha |
Round leaf |
|
|
Lobed leaf |
|
|||
Growth character |
|
|||
Incision |
Depth |
|
||
Size |
|
|||
Lobed leaf number |
|
|||
Leaf apex |
|
|||
Margin of leaf |
|
|||
Leaf base |
|
|||
Leaf vein |
|
|||
Leaf color |
|
|||
Lustre |
|
|||
Coarse leaf |
|
|||
Wrinkle |
|
|||
Size |
Leaf length |
|
||
Leaf width |
|
|||
Trichome |
Leaf surface |
|
||
Leaf back |
|
|||
Leaf vein |
|
|||
Leaf area |
|
|||
Leaf thickness |
|
|||
Tender leaf |
Color |
|
||
Coarse leaf |
|
|||
Trichome |
|
|||
Leaf stalk |
Growth character |
|
||
Length |
|
|||
Width |
|
|||
Color |
|
|||
Trichome |
|
|||
|
|
|||
Stipule |
Morpha |
|
||
Color |
|
|||
Size |
|
|||
References |
Table 7. Investigation of flower and fruit of mulberry germplasm
variety |
Name |
Number |
||
Origin |
District |
Pruning type |
||
Monoecious or dioecious |
Female and male for one flower or not |
|||
Unisexual or bisexual flower |
Flower head |
|||
Female flower |
Catkin |
Morpha |
|
|
Size |
Length |
|
||
Width |
|
|||
Number |
|
|||
Color |
|
|||
Flower number |
|
|||
Flower character |
|
|||
Flower stalk length |
|
|||
|
|
|||
Flower |
Flower cover |
Morpha |
|
|
Size |
|
|||
Trichome |
|
|||
Style length |
|
|||
Stigma |
Morpha |
|
||
Length |
|
|||
Trichome |
|
|||
Bulge |
|
|||
Color |
|
|||
Ovary |
Morpha |
|
||
Size |
|
|||
Male flower |
Catkin |
Morpha |
|
|
Size |
Length |
|
||
Width |
|
|||
Number |
|
|||
Color |
|
|||
Flower number |
|
|||
Flower character |
|
|||
Flower stalk length |
|
|||
|
|
|||
Flower |
Flower cover |
Morpha |
|
|
Size |
|
|||
Trichome |
|
|||
Flower filament |
Morpha |
|
||
Length |
|
|||
Color |
|
|||
Anther |
Morpha |
|
||
Length |
|
|||
Color |
|
|||
Pollen grain size |
|
|||
|
|
|||
Fruit and sorosis |
Fruit |
Structure |
|
|
Size |
|
|||
Fruit stalk |
Color |
|
||
Trichome |
|
|||
sorosis |
Structure |
|
||
Size |
Length |
|
||
Diameter |
|
|||
Color |
|
|||
Taste |
|
|||
Number |
|
|||
Fructification |
|
|||
Drop character |
|
|||
Mature character |
|
|||
|
|
|||
References |
Table 8. Growth duration and economic characters of mulberry germplasm
Variety |
Tree years |
Cut type |
Investigation time |
||
growth |
Budding time |
Budding stage |
|
||
Leaf apex opening stage |
|
||||
Leaf growth day |
1st leaf |
|
|||
2nd leaf |
|
||||
3rd leaf |
|
||||
4th leaf |
|
||||
5th leaf |
|
||||
Flower open day |
Female |
Opening day |
|
||
|
|
||||
|
|
||||
Male |
|
|
|||
|
|
||||
|
|
||||
Spring leaf |
Growth duration |
|
|||
Mature time |
|
||||
Autumn leaf |
Growth duration |
|
|||
New shoot stopping day |
|
||||
Harding day |
|
||||
Leaf yellowed day |
|
||||
|
|
||||
Full year growth duration |
|
||||
Budding ratio (%) |
|
||||
Ratio of growth bud to growth-ended shoot let |
Growth bud |
Percent |
|
||
Length |
|
||||
Size |
|
||||
Leaf number |
|
||||
Growth-ended shoot let |
Percent |
|
|||
Leaf number |
|
||||
|
|
||||
|
|
||||
Shoot |
Single tree |
Shoot number |
|
||
Shoot length for one tree |
|
||||
Single shoot |
Shoot length |
|
|||
Shoot diameter |
|
||||
Ratioof shoot to leaf to fruit |
Shoot |
|
|||
New shoot |
|
||||
Leaf |
|
||||
Fruit |
|
||||
Yield quantity per leaf |
Spring |
Leaf number per kg |
|
||
Weight per leaf |
|
||||
Autumn |
Leaf number per kg |
|
|||
Weight per leaf |
|
||||
Leaf quantity |
Spring |
Shoot let length per meter |
|
||
Yield for one tree |
|
||||
Yield for one hectare |
|
||||
Investigation date |
|
||||
Autumn |
Shoot let length per meter |
|
|||
Yield for one tree |
|
||||
Yield for one hectare |
|
||||
Investigation date |
|
||||
Water content and wilting speed |
Water content |
Spring leaf |
|
||
Autumn leaf |
|
||||
Water Evaporation rate |
After 2 hrs |
|
|||
After 4 hrs |
|
||||
After 6 hrs |
|
||||
After 8 hrs |
|
||||
After 10 hrs |
|
||||
After 12 hrs |
|
||||
References |
Table 9. Resistance to disease and pest of mulberry germplasm
Variety |
Tree old |
Cut type |
Years |
Diseases |
Dwarf disease |
Infection time |
|
Disease ratio |
|
||
Twig blight |
Infection time |
|
|
Disease ratio |
|
||
Dogare blight |
Infection time |
|
|
Disease ratio |
|
||
Bacterial blight |
Infection time |
|
|
Disease ratio |
|
||
Powdery mildew |
Infection time |
|
|
Disease ratio |
|
||
Rust aecidium mori |
Infection time |
|
|
Disease ratio |
|
||
White root rot |
Infection time |
|
|
Disease ratio |
|
||
Violet root rot |
Infection time |
|
|
Disease ratio |
|
||
|
|
||
|
|
||
Pest |
Mulberry small weevil |
Infestation time |
|
Harmful degree |
|
||
Mulberry shoot dall midge |
Infestation time |
|
|
Harmful degree |
|
||
Mulberry leaf roller |
Infestation time |
|
|
Harmful degree |
|
||
Mulberry pyralid |
Infestation time |
|
|
Harmful degree |
|
||
Mulberry sucker |
Infestation time |
|
|
Harmful degree |
|
||
Fall webworm |
Infestation time |
|
|
Harmful degree |
|
||
Mulberry yellow tail moth |
Infestation time |
|
|
Harmful degree |
|
||
Mulberry looper |
Infestation time |
|
|
Harmful degree |
|
||
Mulberry borer |
Infestation time |
|
|
Harmful degree |
|
||
Mulberry thrip |
Infestation time |
|
|
Harmful degree |
|
||
Mites |
Infestation time |
|
|
Harmful degree |
|
||
Natural disasters |
Frost |
Occurrence time |
|
Harmful degree |
|
||
cool |
Occurrence time |
|
|
Harmful degree |
|
||
Drought |
Occurrence time |
|
|
Harmful degree |
|
||
Flooding |
Occurrence time |
|
|
Harmful degree |
|
||
Wind |
Occurrence time |
|
|
Harmful degree |
|
||
|
|
||
|
|
||
References |
Table 10. Comparative study of mulberry growth after pruning in summer
Varieties |
Shoot length (cm) |
Growth rate (%) |
||||||||
Date |
6/10 |
6/30 |
7/20 |
8/9 |
8/29 |
9/18 |
10/28 |
Before 7/30 |
Before 9/8 |
Before 10/28 |
Tong xiang qing |
20.7 |
57.5 |
124.3 |
160.7 |
180.0 |
195.0 |
201.7 |
71.78 |
20.29 |
7.93 |
Bai tiao sang |
19.3 |
64.3 |
113.0 |
149.0 |
161.3 |
18.03 |
189.0 |
70.89 |
17.98 |
11.03 |
He ye bai |
21.1 |
72.7 |
129.0 |
162.0 |
182.3 |
- |
- |
80.33 |
19.67 |
- |
Wu pi sang |
19.0 |
38.5 |
85.0 |
112.0 |
114.3 |
148.7 |
151.9 |
67.76 |
24.34 |
7.90 |
Honh ding sang |
12.0 |
46.0 |
83.0 |
110.0 |
135.0 |
162.0 |
176.0 |
55.68 |
28.97 |
15.35 |
Sheng xian qing |
13.9 |
33.8 |
77.3 |
112.0 |
134.0 |
156.0 |
162.0 |
58.02 |
29.01 |
12.97 |
Wang hai sang |
15.8 |
63.0 |
115.0 |
151.0 |
171.0 |
193.0 |
199.0 |
67.87 |
22.07 |
9.05 |
Juan sang |
21.7 |
64.0 |
99.3 |
125.0 |
144.0 |
157.0 |
162.0 |
68.25 |
25.00 |
6.25 |
Zhen sang |
19.0 |
54.0 |
80.1 |
111.0 |
131.0 |
150.0 |
168.0 |
55.95 |
27.39 |
16.66 |
Wu sang |
26.0 |
73.0 |
126.0 |
175.0 |
207.0 |
231.0 |
252.1 |
61.11 |
30.87 |
19.00 |